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halo tagged proteins  (New England Biolabs)


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    New England Biolabs halo tagged proteins
    Halo Tagged Proteins, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/halo+tagged+proteins/SNAP-Surface+549/pmc12796366-449-4-19
    Average 96 stars, based on 162 article reviews
    halo tagged proteins - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Fluorescent protein and peptide tags alter condensate formation and dynamics in vivo and in vitro
    Article Snippet: .. To label SNAP or Halo tagged proteins in cells, cells were incubated with either 2 μM SNAP-Surface 549 dye (NEB, Cat# S9112S) for 30 min before fixation or 200 nM TMR ligand (Promega, Cat# G8251) for 15 min and washed three times with PB before fixation. ..



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    Image Search Results


    Schematic presentation of the i -GT-ray screening platform coupled with DPS-MS detection method. A , capture antibody and biotinylated plasmid DNA are immobilized on microplates, which is used to couple protein synthesis ( via IVTT system) and capturing on microplate to create a GT-array. B , GT-array is then used for transferase assay using NDP-sugar donors and acceptors. C , products generated by transferase reactions can be detected through DPS-MS. DPS-MS, desalting paper spray-mass spectrometry; GT, glycosyltransferase; i -GT-ray, in vitro GT-array; IVTT, in vitro transcription/translation; NDP, nucleotide diphosphate.

    Journal: The Journal of Biological Chemistry

    Article Title: Streamlining assays of glycosyltransferases activity using in vitro GT-array ( i -GT-ray) platform: Application to family GT37 fucosyltransferases

    doi: 10.1016/j.jbc.2024.105734

    Figure Lengend Snippet: Schematic presentation of the i -GT-ray screening platform coupled with DPS-MS detection method. A , capture antibody and biotinylated plasmid DNA are immobilized on microplates, which is used to couple protein synthesis ( via IVTT system) and capturing on microplate to create a GT-array. B , GT-array is then used for transferase assay using NDP-sugar donors and acceptors. C , products generated by transferase reactions can be detected through DPS-MS. DPS-MS, desalting paper spray-mass spectrometry; GT, glycosyltransferase; i -GT-ray, in vitro GT-array; IVTT, in vitro transcription/translation; NDP, nucleotide diphosphate.

    Article Snippet: GDP or UDP-GLO glycosyltransferase assay, Halo-GST tag fusion protein, and protein expression kit (TNT Quick Coupled Transcription/Translation System) were purchased from Promega.

    Techniques: Plasmid Preparation, Glutathione S-Transferase Assay, Generated, Mass Spectrometry, In Vitro

    Key reagents and resources

    Journal: mBio

    Article Title: SUMOylation of the m6A reader YTHDF2 by PIAS1 promotes viral RNA decay to restrict EBV replication

    doi: 10.1128/mbio.03168-23

    Figure Lengend Snippet: Key reagents and resources

    Article Snippet: Halo-tag protein purification kit , VWR/Promega , Cat #PAG6790.

    Techniques: Magnetic Beads, Conjugation Assay, Protein Purification, Immunoprecipitation, DNA Purification, Construct, Plasmid Preparation